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Image Search Results
Journal: Annals of biomedical engineering
Article Title: Integrin β4 Signaling Promotes Mammary Tumor Cell Adhesion to Brain Microvascular Endothelium by Inducing ErbB2-mediated Secretion of VEGF
doi: 10.1007/s10439-011-0321-6
Figure Lengend Snippet: β4 integrin expression of the ErbB2-transformed mouse mammary tumor cells (Neu-β4-WT and Neu-β4-1355T) expressing either wild type β4 (β4-WT) or β4 signaling defect (β4-1355T), and a normal murine mammary gland (NMuMG) epithelial cell line. Cells were lysed and subjected to immunoblotting with anti-β4 and anti-β-actin. * proteolytic fragment of recombinant human β4. • residual endogenous mouse β4.
Article Snippet:
Techniques: Expressing, Transformation Assay, Western Blot, Recombinant
Journal: Annals of biomedical engineering
Article Title: Integrin β4 Signaling Promotes Mammary Tumor Cell Adhesion to Brain Microvascular Endothelium by Inducing ErbB2-mediated Secretion of VEGF
doi: 10.1007/s10439-011-0321-6
Figure Lengend Snippet: (a) NMuMG, (b) WT and (c) 1355T cell adhesion to bEnd3 cell monolayers. Adhesion was measured 1 hr after seeding under control conditions. The bright spots indicate the adherent fluorescently-labeled cells observed after washing away unattached cells. (d) Comparison of adhesion density (number of adherent cells per unit area of the bEnd3 monolayer) of NMuMG, WT and 1355T cells under control (n=12) and under anti-integrin α6 (n=6) and anti-laminin-5 (n=6) treatment. Anti-mouse IgG was used as a sham control. Cell seeding density of NMuMG, WT and 1355T cells was 2 × 105/ml. The deposit time for both control and treatments was 1 hr. Ten typical fields of 750 μm × 560 μm per run for each cell type were analyzed. *p<0.05, **p<0.01, ***p<0.001.
Article Snippet:
Techniques: Labeling
Journal: Annals of biomedical engineering
Article Title: Integrin β4 Signaling Promotes Mammary Tumor Cell Adhesion to Brain Microvascular Endothelium by Inducing ErbB2-mediated Secretion of VEGF
doi: 10.1007/s10439-011-0321-6
Figure Lengend Snippet: Images of fluorescently-labeled hemidesmosomal marker proteins, β4 integrin (green) and BPAG-1 (red), and the overlay of β4 and BPAG-1 (orange). WT (upper panel) and 1355T (lower panel) tumor cell were allowed to adhere to 10 μg/ml of fibronectin (b, e) and laminin-5 (c, f). Control group adhesion to 0.01% BSA is shown in panels a and d. The amount of β4 integrin and BPAG-1 in tumor cells was quantified based on the pixel numbers of the green labeled area or the red labeled area per μm2, respectively, and related to the total image area, in (g) and (h). The amounts of hemidesmosomes were quantified by the co-localization of β4 integrin and BPAG-1, the orange labeled area, in (i). n=3, *p<0.05, **p<0.01.
Article Snippet:
Techniques: Labeling, Marker